catalog number e el h0109c  (Elabscience Biotechnology)

Journal: Actas Españolas de Psiquiatría

Article Title: Schisandrin A Alleviates Inflammation and Oxidative Stress in Aβ 25-35 -Induced Alzheimer’s Disease in Vitro Model

doi: 10.62641/aep.v52i5.1680

Figure Lengend Snippet: SCH A treatment inhibits A β 25-35 -induced inflammatory injury . (A,B) Enzyme-linked immunosorbent assay (ELISA) was used to assess interleukin (IL)-6, IL-1 β , and tumor necrosis factor (TNF)- α levels. n = 3, *⁣** p < 0.001 vs. control subgroup; # p < 0.05, #⁢# p < 0.01, #⁢#⁢# p < 0.001 vs. A β 25-35 subgroup.

Article Snippet: The cell culture supernatant of each subgroup was collected, and the levels of tumor necrosis factor (TNF)- α (E-EL-H0109c), interleukin-(IL)-1 β (AZ-003-50ug), and IL-6 (21865-1-AP) were evaluated using corresponding ELISA kits (Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: Actas Españolas de Psiquiatría

Article Title: Schisandrin A Alleviates Inflammation and Oxidative Stress in Aβ 25-35 -Induced Alzheimer’s Disease in Vitro Model

doi: 10.62641/aep.v52i5.1680

Figure Lengend Snippet: Extracellular signal-regulated kinase (ERK)/mitogen-activated protein kinase (MAPK) pathway was associated with the mechanism by which SCH A improves the damage caused by A β 25-35 . (A) Western blot analysis was used to determine the levels of ERK1/2 and p-ERK1/2 proteins. n = 3, *⁣** p < 0.001 vs. control subgroup; # p < 0.05, #⁢# p < 0.01 vs. A β 25-35 subgroup. (B) SH-SY5Y cells were treated with ERK activator LM22B-10, and the levels of p-ERK1/2 and ERK1/2 proteins were assessed using Western blot analysis. (C,D) The apoptosis rate of each group was determined using flow cytometry. (E) SOD, MDA, IL-6, and TNF- α levels were examined using corresponding ELISA kits. n = 3, * p < 0.05, ** p < 0.01, *⁣** p < 0.001. GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

Article Snippet: The cell culture supernatant of each subgroup was collected, and the levels of tumor necrosis factor (TNF)- α (E-EL-H0109c), interleukin-(IL)-1 β (AZ-003-50ug), and IL-6 (21865-1-AP) were evaluated using corresponding ELISA kits (Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer’s instructions.

Techniques: Western Blot, Control, Flow Cytometry, Enzyme-linked Immunosorbent Assay